16 35 amplifier system Search Results


mhc iix  (Developmental Studies Hybridoma Bank)
95
Developmental Studies Hybridoma Bank mhc iix
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Mhc Iix, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
mhc iix - by Bioz Stars, 2026-06
95/100 stars
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1 palmitoyl 2 oleoylphosphatidylcholine popc  (Avanti Polar)
99
Avanti Polar 1 palmitoyl 2 oleoylphosphatidylcholine popc
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
1 Palmitoyl 2 Oleoylphosphatidylcholine Popc, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 palmitoyl 2 oleoylphosphatidylcholine popc/product/Avanti Polar
Average 99 stars, based on 1 article reviews
1 palmitoyl 2 oleoylphosphatidylcholine popc - by Bioz Stars, 2026-06
99/100 stars
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magnetic coil figure-of-eight standard double  (Magstim Company)
90
Magstim Company magnetic coil figure-of-eight standard double
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Magnetic Coil Figure Of Eight Standard Double, supplied by Magstim Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/magnetic coil figure-of-eight standard double/product/Magstim Company
Average 90 stars, based on 1 article reviews
magnetic coil figure-of-eight standard double - by Bioz Stars, 2026-06
90/100 stars
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hpv rna probes targeting 18 hpv types  (MyGenostics Inc)
90
MyGenostics Inc hpv rna probes targeting 18 hpv types
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Hpv Rna Probes Targeting 18 Hpv Types, supplied by MyGenostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hpv rna probes targeting 18 hpv types/product/MyGenostics Inc
Average 90 stars, based on 1 article reviews
hpv rna probes targeting 18 hpv types - by Bioz Stars, 2026-06
90/100 stars
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rabbit anti-cd206 antibody  (ABclonal Biotechnology)
90
ABclonal Biotechnology rabbit anti-cd206 antibody
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Rabbit Anti Cd206 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-cd206 antibody/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
rabbit anti-cd206 antibody - by Bioz Stars, 2026-06
90/100 stars
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pooled human liver microsomes (pooled male female  (Cyprotex Discovery)
90
Cyprotex Discovery pooled human liver microsomes (pooled male female
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Pooled Human Liver Microsomes (Pooled Male Female, supplied by Cyprotex Discovery, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pooled human liver microsomes (pooled male female/product/Cyprotex Discovery
Average 90 stars, based on 1 article reviews
pooled human liver microsomes (pooled male female - by Bioz Stars, 2026-06
90/100 stars
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dipalmitoyl sn glycero 3 phosphoethanolamine n  (Croda International Plc)
95
Croda International Plc dipalmitoyl sn glycero 3 phosphoethanolamine n
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Dipalmitoyl Sn Glycero 3 Phosphoethanolamine N, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dipalmitoyl sn glycero 3 phosphoethanolamine n/product/Croda International Plc
Average 95 stars, based on 1 article reviews
dipalmitoyl sn glycero 3 phosphoethanolamine n - by Bioz Stars, 2026-06
95/100 stars
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hmo11  (Elicityl Inc)
90
Elicityl Inc hmo11
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Hmo11, supplied by Elicityl Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hmo11/product/Elicityl Inc
Average 90 stars, based on 1 article reviews
hmo11 - by Bioz Stars, 2026-06
90/100 stars
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superdex 200 hiload 16 600 column  (Cytiva Europe)
97
Cytiva Europe superdex 200 hiload 16 600 column
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Superdex 200 Hiload 16 600 Column, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superdex 200 hiload 16 600 column/product/Cytiva Europe
Average 97 stars, based on 1 article reviews
superdex 200 hiload 16 600 column - by Bioz Stars, 2026-06
97/100 stars
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microwell 1.5 coverglasss  (MatTek)
90
MatTek microwell 1.5 coverglasss
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Microwell 1.5 Coverglasss, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microwell 1.5 coverglasss/product/MatTek
Average 90 stars, based on 1 article reviews
microwell 1.5 coverglasss - by Bioz Stars, 2026-06
90/100 stars
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chromogenic mrsa selective agar  (Thermo Fisher)
99
Thermo Fisher chromogenic mrsa selective agar
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Chromogenic Mrsa Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chromogenic mrsa selective agar/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
chromogenic mrsa selective agar - by Bioz Stars, 2026-06
99/100 stars
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trypsin sequence grade  (Promega)
90
Promega trypsin sequence grade
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Trypsin Sequence Grade, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
trypsin sequence grade - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against MHC-I (purple), MHC-IIb (cyan), MHC-IIa (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared

Journal: Cell Death & Disease

Article Title: The histone code reader Spin1 controls skeletal muscle development

doi: 10.1038/cddis.2017.468

Figure Lengend Snippet: Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against MHC-I (purple), MHC-IIb (cyan), MHC-IIa (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared

Article Snippet: For immunofluorescence staining the following primary antibodies were used: SPIN1(5865) 1 μ g/ml; Pax7 (PAX7, DSHB, batch 7/2/15) 2 μ g/ml; Tcf4 (6H5-3, Millipore, Darmstadt, Germany, 05-511, batch 2155406) 10 μ g/ml; Ankrd1 (Proteintech Group, Manchester, UK, 11427-1-AP, batch 1951) 1:100; Ankrd2 (Proteintech Group, 11821-1-AP, batch 7649) 1:100; dystrophin (Abcam, Cambridge, UK, ab15277, batch GR226781-6) 1:500; MHC-I (NOQ7.5.4D, Sigma, Munich, Germany, M8421, batch 035M4792V) 1:2000; MHC-IIa (SC-71, DSHB, batch 4/7/16) 1:10; MHC-IIx (6H1, DSHB, batch 3/3/16) 1:6; MHC-IIb (BF-F3, DSHB, batch 5/12/16) 1:20; MCH, skeletal, fast (MY-32, Sigma, M4276, batch 083M4790V) 1:1000; GNZ (anti-GFP, Abcam, ab13970, batch GR236651) 1:1000; normal rabbit IgG (Santa Cruz, Heidelberg, Germany, sc-2027).

Techniques: Control, Muscles, Staining, Immunofluorescence, Comparison